Melt Curve Fitting and Melt Shift Analysis

Documentation for package ‘InflectSSP’ version 1.6

Correction	This function corrects the normalized abundance of each protein using a correction constant that is calculated in this function. The correction constant is determined using the difference between actual and predicted fit at the proteome level.
CurveFit1	This function determines the 4 parameter or 3 parameter log fit for the proteome level curve.
CurveFit2	This function determines the best curve fit for each protein using the data post correction and also determines the R squared for each curve fit
Import	This function imports data that will be analyzed in downstream functions.
InflectSSP	This function is the primary function that calls other functions in the program.
MeltCalc	This function determines melt shifts for all proteins that meet quality criteria and also determines the melt shift p-values
Normalize	This function normalizes the abundance values to that measured at the lowest temperature
Quantify	This function determines the median abundance value across the proteome for all experiments together
ReportDataMelts	This function generates results from the Inflect function after applying criteria input from the user
ReportSTRING	This function generates a STRING based network using the significant melt shifts from analysis